Laboratory of Computational and Quantitative Biology (LCQB)
UMR 7238 CNRS - Sorbonne Université
You are here
Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.
| Title | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae. |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Boyer, J, Badis, G, Fairhead, C, Talla, E, Hantraye, F, Fabre, E, Fischer, G, Hennequin, C, Koszul, R, Lafontaine, I, Ozier-Kalogeropoulos, O, Ricchetti, M, Richard, G-F, Thierry, A, Dujon, B |
| Journal | Genome Biol |
| Volume | 5 |
| Issue | 9 |
| Pagination | R72 |
| Date Published | 2004 |
| ISSN | 1465-6914 |
| Keywords | Cloning, Molecular, DNA, Fungal, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Lethal, Genes, Suppressor, Genome, Fungal, Open Reading Frames, Phenotype, Reading Frames, Saccharomyces cerevisiae, Transfection, Transgenes |
| Abstract | We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast. |
| DOI | 10.1186/gb-2004-5-9-r72 |
| Alternate Journal | Genome Biol. |
| PubMed ID | 15345056 |
| PubMed Central ID | PMC522879 |
