Laboratory of Computational and Quantitative Biology (LCQB)
UMR 7238 CNRS - Sorbonne Université
You are here
Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.
| Title | Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697. |
| Publication Type | Journal Article |
| Year of Publication | 1996 |
| Authors | Berger, F, Fischer, G, Kyriacou, A, Decaris, B, Leblond, P |
| Journal | FEMS Microbiol Lett |
| Volume | 143 |
| Issue | 2-3 |
| Pagination | 167-73 |
| Date Published | 1996 Oct 1 |
| ISSN | 0378-1097 |
| Keywords | Base Sequence, Chromosome Mapping, Chromosomes, Bacterial, Cloning, Molecular, DNA Primers, DNA, Bacterial, Genes, Bacterial, Multigene Family, Operon, Polymerase Chain Reaction, RNA, Bacterial, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Streptomyces |
| Abstract | Streptomyces ambofaciens DSM40697 possess six rRNA gene clusters which can be distinguished by probing BfrI-digested total DNA with the PCR-amplified l6S-23S ribosomal genet internal transcribed spacer. The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA. For five of the six ribosomal gene sets, the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus. |
| Alternate Journal | FEMS Microbiol. Lett. |
| PubMed ID | 8837469 |
